the effect of seminal plasma on the quality of coated ram frozen-thawed spermatozoa

two experiments were designed to examine the effects of crude seminal plasma (csp) exposure ofejaculated and epididymal spermatozoa before freezing. experiment 1, two consecutive ejaculates werecollected (n=4) within 5 min, the second one was carried out in a tube containing fiser extender (coatedspermatozoa); by centrifugation, seminal plasma was removed from coated ejaculates. the pellets were split into three parts and 0, 50 and 100% csp (v/v) was added and they were frozen. samples were thawed and incubated at 37°c for 6 h. the highest progressive motility (10.41%) and the highest hypo-osmotic responses (12.62%) were found for the coated spermatozoa without csp at 6 (p